Composite

Part:BBa_K2384006:Design

Designed by: Haiyang Chen   Group: iGEM17_FAFU-CHINA   (2017-10-21)


Pveg+RBS+CspC+6*HIS+T1 terminator+T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CDS sequence optimization in BBa_K538004 is better suited for expression in Bacillus megaterium and we add a 6*His tag at the C end.After that, the promoter (BBa_K143012) and RBS (BBa_K090505) were combined.


Source

This gene is a cold resistant gene from the Arctic cryophile Polaribacter irgensii from BBa_K538004 . This gene is composed of synthetic biology company.

References